cmv a b polya hdvr bgh fragment  (New England Biolabs)

Journal: The FASEB Journal

Article Title: Astrocytic FABP7 Alleviates Depression‐Like Behaviors of Chronic Unpredictable Mild Stress Mice by Regulating Neuroinflammation and Hippocampal Spinogenesis

doi: 10.1096/fj.202403417RR

Figure Lengend Snippet: Effects of FABP7 overexpression on depression‐like behaviors and protein expressions in HP of the CUMS mice. (A) Schematic of the experimental procedure and schedule. (B) Representative fluorescent image illustrating the expression of green fluorescent protein (GFP). (C) Number of horizontal motions in the OFT. (D) Number of vertical motions in the OFT. (E) Immobility time in the FST. (F) Immobility time in the TST. (G) Sucrose preference in the SPT. (H) Changes in the protein expression of FABP7 (a, c) and Cav‐1 (b, d) in the HP of CUMS mice. Data were presented as mean ± SEM ( n = 5–14 per group) and normalized to the control group (CON+AAV‐GFP) for analysis. Two‐way ANOVA with Tukey's honestly significant difference (HSD), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The control virus (ov‐control: gfaABC1D promoter‐MCS‐EGFP‐WPRE‐bGH PolyA, 1.78E+13 v.g/mL, Genechem, Shanghai, China) or FABP7 construct (ov‐FABP7: gfaABC1D promoter‐FABP7‐EGFP‐WPRE‐bGH PolyA, 2.13E+13 v.g/mL, Genechem, Shanghai, China) was delivered bilaterally through microinjection in the hippocampus (HP, −1.8 mm anterior–posterior, ±1.5 mm medial‐lateral, and‐2.0 mm dorsal‐ventral from bregma; Figure ) at a rate of 0.5 μL/min, with a unilateral volume of 1 μL.

Techniques: Over Expression, Expressing, Control

Journal: The FASEB Journal

Article Title: Astrocytic FABP7 Alleviates Depression‐Like Behaviors of Chronic Unpredictable Mild Stress Mice by Regulating Neuroinflammation and Hippocampal Spinogenesis

doi: 10.1096/fj.202403417RR

Figure Lengend Snippet: Effects of CUMS on depression‐like behavior and the expression of FABP7 and Cav‐1. (A) Schematic of the experimental procedure and schedule. (B) Number of horizontal motions in the OFT. (C) Number of vertical motions in the OFT. (D) Immobility time in the FST. (E) Immobility time in the TST. (F) Sucrose preference in the SPT. The effect of CUMS on protein expression of FABP7 (G) and Cav‐1 (H) in the HP. (I) A representative image of co‐immunoprecipitation. (J) Representative immunofluorescence double‐labeling of FABP7 and GFAP (a) /NeuN (b). Data were presented as mean ± SEM ( n = 6–9) and normalized to the control group (CON) for analysis. Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The control virus (ov‐control: gfaABC1D promoter‐MCS‐EGFP‐WPRE‐bGH PolyA, 1.78E+13 v.g/mL, Genechem, Shanghai, China) or FABP7 construct (ov‐FABP7: gfaABC1D promoter‐FABP7‐EGFP‐WPRE‐bGH PolyA, 2.13E+13 v.g/mL, Genechem, Shanghai, China) was delivered bilaterally through microinjection in the hippocampus (HP, −1.8 mm anterior–posterior, ±1.5 mm medial‐lateral, and‐2.0 mm dorsal‐ventral from bregma; Figure ) at a rate of 0.5 μL/min, with a unilateral volume of 1 μL.

Techniques: Expressing, Immunoprecipitation, Immunofluorescence, Labeling, Control

Journal: The FASEB Journal

Article Title: Astrocytic FABP7 Alleviates Depression‐Like Behaviors of Chronic Unpredictable Mild Stress Mice by Regulating Neuroinflammation and Hippocampal Spinogenesis

doi: 10.1096/fj.202403417RR

Figure Lengend Snippet: Effects of FABP7 overexpression on GFAP and inflammatory factor protein expression in HP of the CUMS mice. (A) Representative Western blots for GFAP, IL‐1β, and IL‐6. (B–D) Quantitative analysis of GFAP (B), IL‐1β (C), and IL‐6 (D) proteins in the HP. Data were presented as mean ± SEM ( n = 5–6) and normalized to the control group (CON+AAV‐GFP) for analysis. Two‐way ANOVA with Tukey's honestly significant difference (HSD), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The control virus (ov‐control: gfaABC1D promoter‐MCS‐EGFP‐WPRE‐bGH PolyA, 1.78E+13 v.g/mL, Genechem, Shanghai, China) or FABP7 construct (ov‐FABP7: gfaABC1D promoter‐FABP7‐EGFP‐WPRE‐bGH PolyA, 2.13E+13 v.g/mL, Genechem, Shanghai, China) was delivered bilaterally through microinjection in the hippocampus (HP, −1.8 mm anterior–posterior, ±1.5 mm medial‐lateral, and‐2.0 mm dorsal‐ventral from bregma; Figure ) at a rate of 0.5 μL/min, with a unilateral volume of 1 μL.

Techniques: Over Expression, Expressing, Western Blot, Control

Journal: The FASEB Journal

Article Title: Astrocytic FABP7 Alleviates Depression‐Like Behaviors of Chronic Unpredictable Mild Stress Mice by Regulating Neuroinflammation and Hippocampal Spinogenesis

doi: 10.1096/fj.202403417RR

Figure Lengend Snippet: Effects of FABP7 overexpression on co‐localization of FABP7 and GFAP in CA1, CA3 and DG of the HP of the CUMS‐treated mice. (A, B) Representative images of FABP7 and GFAP of CA1, CA3, and DG regions of HP. Scale bar = 50 μm. (C–H) Quantitation of FABP7 + ‐GFAP + cell in the HP. Data were presented as mean ± SEM ( n = 3 per group) and normalized to the control group (CON/CON+AAV‐GFP) for analysis. Student's t test, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The control virus (ov‐control: gfaABC1D promoter‐MCS‐EGFP‐WPRE‐bGH PolyA, 1.78E+13 v.g/mL, Genechem, Shanghai, China) or FABP7 construct (ov‐FABP7: gfaABC1D promoter‐FABP7‐EGFP‐WPRE‐bGH PolyA, 2.13E+13 v.g/mL, Genechem, Shanghai, China) was delivered bilaterally through microinjection in the hippocampus (HP, −1.8 mm anterior–posterior, ±1.5 mm medial‐lateral, and‐2.0 mm dorsal‐ventral from bregma; Figure ) at a rate of 0.5 μL/min, with a unilateral volume of 1 μL.

Techniques: Over Expression, Quantitation Assay, Control

Journal: The FASEB Journal

Article Title: Astrocytic FABP7 Alleviates Depression‐Like Behaviors of Chronic Unpredictable Mild Stress Mice by Regulating Neuroinflammation and Hippocampal Spinogenesis

doi: 10.1096/fj.202403417RR

Figure Lengend Snippet: Effects of FABP7 overexpression on spinogenesis in HP of the CUMS mice. (A–C) Representative Western blots for NeuN (A), BDNF (B), PSD95, GluA1, and synapsin (C). (D–H) Quantitative analysis of NeuN (D), BDNF (E), PSD95 (F), GluA1 (G), and synapsin (H) proteins in the HP ( n = 6 per group). (I–K) Representative images of dendritic spines from the HP CA1 (I), CA3 (J), and DG (K) subregions. Scale bars: 10 μm. (L–N) Quantitative analysis of dendritic spine density in the HP CA1 (L), CA3 (M), and DG (N) subregions ( n = 3). Data were presented as mean ± SEM and normalized to the control group (CON+AAV‐GFP) for analysis. Two‐way ANOVA with Tukey's honestly significant difference (HSD), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The control virus (ov‐control: gfaABC1D promoter‐MCS‐EGFP‐WPRE‐bGH PolyA, 1.78E+13 v.g/mL, Genechem, Shanghai, China) or FABP7 construct (ov‐FABP7: gfaABC1D promoter‐FABP7‐EGFP‐WPRE‐bGH PolyA, 2.13E+13 v.g/mL, Genechem, Shanghai, China) was delivered bilaterally through microinjection in the hippocampus (HP, −1.8 mm anterior–posterior, ±1.5 mm medial‐lateral, and‐2.0 mm dorsal‐ventral from bregma; Figure ) at a rate of 0.5 μL/min, with a unilateral volume of 1 μL.

Techniques: Over Expression, Western Blot, Control

Journal: The FASEB Journal

Article Title: Astrocytic FABP7 Alleviates Depression‐Like Behaviors of Chronic Unpredictable Mild Stress Mice by Regulating Neuroinflammation and Hippocampal Spinogenesis

doi: 10.1096/fj.202403417RR

Figure Lengend Snippet: Effects of FABP7 overexpression on BBB stability‐related proteins in HP of the CUMS mice. (A, B) Representative Western blots for Claudun‐5, occludin, AQP4 (A), and LRP1 (B) in the HP. (C–F) Quantitative analysis of Claudun‐5 (C), occludin (D), AQP4 (E), and LRP1 (F) proteins in the HP. Data were presented as mean ± SEM ( n = 5–6 per group) and normalized to the control group (CON+AAV‐GFP) for analysis. Two‐way ANOVA with Tukey's honestly significant difference (HSD), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The control virus (ov‐control: gfaABC1D promoter‐MCS‐EGFP‐WPRE‐bGH PolyA, 1.78E+13 v.g/mL, Genechem, Shanghai, China) or FABP7 construct (ov‐FABP7: gfaABC1D promoter‐FABP7‐EGFP‐WPRE‐bGH PolyA, 2.13E+13 v.g/mL, Genechem, Shanghai, China) was delivered bilaterally through microinjection in the hippocampus (HP, −1.8 mm anterior–posterior, ±1.5 mm medial‐lateral, and‐2.0 mm dorsal‐ventral from bregma; Figure ) at a rate of 0.5 μL/min, with a unilateral volume of 1 μL.

Techniques: Over Expression, Western Blot, Control